Uji Barfoed

Tujuan : Membedakan Monosakarida dan Disakarida Prinsip : Ion Cu 2+  (dari pereaksi Barfoed) dalam suasana asam akan direduksi lebih cepat ...

Tujuan : Membedakan Monosakarida dan Disakarida
Prinsip : Ion Cu2+ (dari pereaksi Barfoed) dalam suasana asam akan direduksi lebih cepat oleh gula reduksi monosakarida daripada disakarida dan menghasilkan endapan Cu2O berwarna merah bata

Alat dan bahan:

  1. Larutan sempel
  2. Pereaksi Barfoed : 48gr Cu asetat dalam 80ml aquades mendidih + asam laktat 8,5% sebanyak 50ml. dalam keadaan panas larutan dikocok sampai endapan larut dan kemudian diencerkan hingga 1000ml lalu disaring.
  3. Larutan Fosfomolibdat
  4. Penangas air
  5. Tabung Reaksi
  6. Stop watch
  7. Penjepit tabung
  8. Pipet tetes
Prosedur Kerja
  1. Masukan 1ml pereaksi barfoed kedalam tabung reaksi
  2. Tambah 5 tetes sempel lalu homogenkan
  3. Panaskan selama 3 menit lalu dinginkan
  4. Tambahkan 1ml larutan fosfomolibdat
  5. Baca hasil
Interpretasi Hasil
  • Monosakarida = terbentuk warna biru tua 
  • Diisakarida      = terbentuk warna biru muda


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Laboratory Information Service: Uji Barfoed
Uji Barfoed
Laboratory Information Service
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